The crystal structure for the C-gate closed conformation of human topoisomerase IIalpha (hTopoIIalpha) is unavailable. Interestingly, most of the anti-hTopoIIalpha interacts with the hTopoIIalpha in its C-closed conformation. This structure represents the homology modeled C-closed conformation of enzyme hTopoIIalpha, in the absence of DNA. The structural domains which are modeled in this structure are the central domain and C-terminal domain. The structural dynamics of the DNA-binding domain in the absence of DNA are evident in this structure.