The enzyme human Topoisomerase IIalpha (hTopoIIalpha) completes the DNA topoisomerisation cycle with the hydrolysis of both the ATP molecules to two ADP molecules. In this structure, the ATP-binding sites are occupied with two ADP molecules. In this structure, the contact points between the two subunits are reduced as compared to that in the ATP-bound state of ATPase domain. This structure can be used to understand the molecular recognition interactions of ADP with hTopoIIalpha.